http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010508831-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0018 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54333 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 2007-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2010-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2010508831-A |
| titleOfInvention | Method for purifying at least one target substance for identification purposes |
| abstract | Disclosed is a method for the purification of at least one target substance present or formed in a cell culture medium for identification purposes and when the cells are cultured. In this method, magnetic particles (ie, beads) that selectively bind the target substance to the functionalized surface are added to the cell culture medium, and the particles to which the target substance is bound are selected from the cell culture medium by applying a magnetic field. It is. This method is characterized by the following steps: providing a serum replacement obtained from natural serum and having no or substantially no low molecular weight substance with a maximum mass of 60 kDa, in particular a maximum mass of 10 kDa Adding a serum replacement to a cell culture medium that already contains cells or to which cells are added; culturing the cells in a cell culture medium with an increased content of the serum replacement; cells formed during culture Separating at least some of the culture supernatant; filtering the cell culture supernatant by ultrafiltration to obtain an unpermeate; functionalized surface of the bead up to 10 side chains (ie 10 generations) Supplying beads to contain a plurality of dendrimers (where the terminal portion of the final generation of each dendrimer is denatured); mixing the beads and impermeate in a buffer to obtain a mixture Step of selecting the magnetic beads magnetically from and mixtures thereof; retentate target substance contained in the culturing step is fixed to the bead. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016535161-A |
| priorityDate | 2006-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 114.