| abstract |
The present invention provides chimeric G proteins, expression constructs thereof, yeast cells expressing such constructs, and methods of making and using them. A C- terminal 10 yeast G was modified by substitution of at least 3 amino acid positions in another different amino acid in the amino acid α (Gpa1p) chimeric G alpha protein having an amino acid sequence, the chimeric G alpha protein Interacting with a receptor comprising the transformed yeast cell comprising, and contacting the compound of interest with said yeast cell, observing the growth response of the cell or observing the production of a reporter gene product A screening method for compounds that can be used. [Selection] Figure 5 |