| abstract |
An engineered eukaryotic host that can be used to express and target any desired gene involved in glycosylation for the production of therapeutic glycoproteins in mammals (eg, humans). Provide cells. Methods for producing human-like glycoproteins in non-human eukaryotic host cells having oligosaccharides modified by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases, and cells in eukaryotic hosts Nucleic acid molecules and combinatorial libraries that can be used to target and express mammalian enzyme activities involved in glycosylation of internal compartments. [Selection figure] None |