| abstract |
[PROBLEMS] To improve the stability of a visualization probe (fluorescence / bioluminescence imaging probe) that detects and measures a three-dimensional structure change caused by binding of a ligand of a conventional information recognition protein by bioluminescence or fluorescence, and has a detection limit, a detection range, Improve performance such as selectivity and sensitivity. [MEANS FOR SOLVING PROBLEMS] A corepressor-activating peptide, for example, a peptide containing an “LXXIIXXXL” motif derived from a corepressor sequence, was introduced as an anchor peptide into a conventional fluorescent / bioluminescent probe. In addition, the permutation order of each element is significantly recombined using circular permutation (CP). [Selection] Figure 1A |