http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010158256-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ee1c19da359446fb5c4f0458a57b783d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2010-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8401dfc55e43cffb49981913685442c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_711d90023e925a32b51c23969b0c3345 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b54cc780718c172351b956f3c88f643f |
| publicationDate | 2010-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2010158256-A |
| titleOfInvention | Yeast transformation method |
| abstract | The present invention provides a yeast transformation method that does not require adjustment of competent cells and can easily and easily treat a large number of strains. SOLUTION: It consists of a buffer solution containing DNA for transformation, carrier DNA, polyethylene glycol and lithium salt, 0.5 to 10 μg of DNA for transformation per 100 μL, and 20 times mass of carrier DNA relative to the DNA for transformation. 1 × 10 7 or more yeast cells cultured on SD solid medium are added to the treatment solution containing the above, and incubated at 25 to 35 ° C. for 30 minutes or more. A method for producing a transformant by transforming with a DNA for transformation. [Selection] Figure 1 |
| priorityDate | 2010-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.