| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8dc1442e8263a086103a8b2a6b9753f9 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0652
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-24151 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-275
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-285
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0652 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-275
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-285
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P37-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-869 |
| filingDate |
2010-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5317a6208d0c8c7e7c67b47989211876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f24a8564ff49b58128de926c11f565aa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f28856dee73188de9d4e1f20b7ba747 |
| publicationDate |
2010-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2010148522-A |
| titleOfInvention |
Method for culturing primary cells under serum-free conditions and method for amplifying virus |
| abstract |
【Task】 Provided is a method that allows culture of primary cells (especially primary avian cells) in a serum-free medium, which allows (I) growth of cells in log phase and (II) maintenance of cells in stationary phase thing. [Solution] The present invention relates to a method for culturing primary cells. The primary cells are cultured in a serum-free medium containing a factor selected from the group consisting of growth factors and adhesion factors. This primary cell culture method can be one step of a method of amplifying a virus such as poxvirus. In this virus amplification method, primary cells are cultured in a serum-free medium containing a factor selected from the group consisting of growth factors and adhesion factors. The cells are then infected with the virus and the infected cells are cultured in serum-free medium until progeny virus is produced. [Selection figure] None |
| priorityDate |
2002-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |