| abstract |
The present invention provides a novel β-fructofuranosidase having high stability at a high temperature of 50 to 65 ° C. A β-fructofuranosidase comprising the modified amino acid sequence described in (A) to (C) below. (A) A modified amino acid sequence in which at least one amino acid of threonine at position 47, serine at position 200, phenylalanine at position 447, phenylalanine at position 470, and proline at position 500 is substituted in a specific amino acid sequence. (B) In addition to substitution of at least one amino acid selected from the modified amino acid sequence A, at least one mutation selected from the group consisting of deletion, substitution, inversion, addition and insertion of one to several amino acids A modified amino acid sequence B constituting a protein having β-fructofuranosidase activity. (C) A modified amino acid sequence C constituting a protein having 70% or more homology with the modified amino acid sequence A and having β-fructofuranosidase activity. [Selection figure] None |