| abstract |
【Task】 To provide a method for selecting a protease or mutant protease having a desired substrate specificity and activity. [Solution] Candidate and modified proteases with substrates that capture the protease by forming a stable complex upon cleavage of the substrate, such as serpin, alpha macroglobulin or p35 family protein or modified serpin and modified p35 family member or modified alpha macroglobulin By contacting them, the above problems are solved by screening them and identifying modified proteases having altered functions. [Selection] Figure 1 |