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filingDate 2008-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4b3f089574677b44ef258c855e398c5
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publicationDate 2010-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2010063373-A
titleOfInvention Monomer-type streptavidin mutant and its production method
abstract Disclosed is a streptavidin mutant in which a lysine residue at a specific position in monomeric streptavidin is modified and has a binding activity to biotin. At least one selected from lysine residues of Nos. 5, 8, 81, 122, 133, 135, 145 and 146 of monomeric streptavidin represented by a specific amino acid sequence is an aliphatic amino acid or aromatic A streptavidin variant substituted with an amino acid; a DNA molecule encoding the amino acid sequence of the streptavidin variant; a recombinant vector comprising the DNA molecule; a transformant comprising the recombinant vector; and the transformation A method for producing a streptavidin mutant, comprising culturing a body and collecting the streptavidin mutant from the culture. [Selection figure] None
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