| abstract |
A method and composition for making thermolysin in a stabilized form in an aqueous solution. The method includes a first step (P) of mixing a solid preparation comprising thermolysin and an aqueous solvent to produce a first solution, wherein the first solution comprises (i) pH 4.5 A buffer salt capable of maintaining a pH in the range of ~ 9, (ii) one or more salts, and (iii) one or more comprising a thermolysin and the buffer salt (s) in the first solution The concentration of the salt in the range from about 0.1 mM to 500 mM, and the method is carried out by adding a measured amount of NaCl, NaBr, NaNO 3 , NaI, KCl, LiCl, MgCl 2 , CaCl 2 to the first solution. And adding a further salt selected from the group consisting of and a mixture thereof to dissolve the additional salt, thereby preparing a second solution in which the dissolved thermolysin is present in a stabilized form (Q) In addition. [Selection figure] None |