| abstract |
Lipid: nucleic acid complexes with extended shelf life and high in vivo transfection activity after intravenous injection, and methods for preparing such complexes. A nucleic acid is contacted with an organic polycation to produce a concentrated nucleic acid, and the concentrated nucleic acid is combined with a lipid containing an amphiphilic cationic lipid to form a lipid: nucleic acid complex. Furthermore, it is stabilized by adding a hydrophilic polymer bound to hydrophobic side chains. The complex is also specific for a particular cell by incorporating a targeting moiety such as a Fab ′ fragment attached to a hydrophilic polymer. Furthermore, it is engineered to contain a binding protein in which a hydrophilic polymer domain and microparticles are bound to a linker molecule having a hydrophobic domain capable of stable binding, or a lipid moiety that enables stable binding between the hydrophilic domain and microparticles. Lipid microparticles with chemically processed proteins. [Selection figure] None |