Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6dd45aa981988e937877aa9ed1069370 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00637 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00621 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00626 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0061 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00612 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00608 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
filingDate |
2009-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe51d0c9fd9c631fdf46f74a4a706400 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_349ca160053e0487c2cdd0621d0aec36 |
publicationDate |
2010-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-2010011872-A |
titleOfInvention |
Methods for fragmenting, labeling and immobilizing nucleic acids |
abstract |
Disclosed is a novel method and kit for labeling and / or fragmenting and / or immobilizing polynucleotides on a substrate. The present invention relates to a method for fragmentation and / or labeling and / or immobilization of nucleic acids comprising nucleic acids labeled and / or cleaved and / or immobilized at abasic sites. For example, in the present invention, a method for labeling a polynucleotide, comprising: (a) a step of producing a polynucleotide containing the non-canonical nucleotide; and (b) an enzyme capable of cleaving the base portion of the non-canonical nucleotide. Cleaving the base portion of the non-common nucleotide from the synthetic polynucleotide to produce an abasic site, and (d) labeling the polynucleotide or the polynucleotide fragment at the abasic site, And thereby making a labeled polynucleotide is provided. [Selection figure] None |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015100332-A |
priorityDate |
2002-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |