| abstract |
[PROBLEMS] To provide a human artificial chromosome vector that is stably retained in a cell, can easily insert a large-sized foreign gene, and can be introduced into the cell, and a method for producing the same. The present invention comprises a human chromosome 21 fragment from which the distal long arm and / or distal short arm is deleted, and a site-specific recombinase recognition site is inserted proximal to the long arm and / or the proximal short arm. Human artificial chromosome (HAC) vector and method for producing the same. A method for introducing foreign DNA using a human artificial chromosome vector, a method for producing cells expressing foreign DNA, and a method for producing proteins. [Selection figure] None |