Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate |
2009-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02d23a068334f716ed9da17409e97532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99bf905fb2cead96004c52c8cb0433b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27615c3ee60029b9d42e9987d59eb240 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7de306cfe34a12948633a627e2c43ac1 |
publicationDate |
2010-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-2010004884-A |
titleOfInvention |
Improved method for preventing carryover contamination in nucleic acid amplification technology |
abstract |
[PROBLEMS] To prevent carryover contamination in a nucleic acid amplification reaction. An improved method for preventing carryover contamination of an amplification reaction includes the steps of treating uracil-containing DNA with uracil-N-DNA glycosylase and heating the DNA in the presence of a polyamine such as spermidine, spermine, and the like. concern. Alternatively, after treatment with uracil-N-DNA glycosylase, the reaction is further incubated with an enzyme having AP lyase activity. [Selection figure] None |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017518048-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017043114-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10683540-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2017043114-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016540517-A |
priorityDate |
2008-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |