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filingDate 2007-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2009-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2009537013-A
titleOfInvention Antigen capture anti-dengue IgA ELISA (ACA-ELISA) for detection of flavivirus specific antibodies
abstract An antigen capture IgA enzyme immunoassay (ACA-ELISA) for the detection of anti-flavivirus IgA was developed. The assay uses a flavivirus lytic antigen, preferably a dengue lytic antigen captured by a monoclonal antibody. Anti-flavivirus IgA captured from the test serum is preferably detected using a rabbit anti-IgA labeled with a reporter group such as horseradish peroxidase (HRP). This assay was found to be at least 8 times more sensitive than the anti-human IgA capture ELISA (AAC-ELISA). The ACA-ELISA based on either serum or saliva was found to be more sensitive and rapid compared to the “golden rule” anti-dengue IgM detection technique. This can be used as a diagnostic tool for dengue confirmation in the early stage of infection. [Selection figure] None
priorityDate 2006-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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