http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009535054-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2006-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2009535054-A |
| titleOfInvention | Method for amplifying an unknown DNA sequence adjacent to a known sequence |
| abstract | The present invention uses a DNA walking annealing control primer (DW-ACP) and a first target-specific primer (TSP) that can be hybridized to one position of a known nucleotide sequence to convert an unknown nucleotide sequence into a first sequence. A method for amplifying an unknown nucleotide sequence adjacent to a known nucleotide sequence, comprising the step (a) of performing a primary amplification, wherein the step (a) comprises the following steps: (a-1) (i) a degenerate random nucleotide sequence hybridized to the unknown nucleotide sequence, and (ii) a hybridized nucleotide sequence substantially complementary to one position of the unknown nucleotide sequence, Performing a first stage amplification of the unknown nucleotide sequence at a first annealing temperature, wherein a first degenerate DW-ACP extension product is generated, wherein the first annealing temperature is a first degenerative DW-ACP extension product, -Make ACP act as a primer. And (a-2) performing a second-stage amplification of the amplification product generated in step (a-1) at a second annealing temperature higher than the first annealing temperature. Then, using the first degenerate DW-ACP and the first TSP used in the step (a-1), the first amplification product is obtained under the condition that each primer is annealed with the target nucleotide sequence. Generated. [Selection] Figure 1A |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020182453-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020503838-A |
| priorityDate | 2006-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 217.