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filingDate 2007-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2009-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2009530607-A
titleOfInvention Competitive enzyme-linked immunosorbent assay (C-ELISA) for flavivirus-specific antibody detection
abstract A competitive enzyme-linked immunosorbent assay (C-ELISA) using flavivirus member-specific immunological substances was developed to detect specific antibodies to flavivirus members that are indicative of flavivirus exposure. This test is based on competition for epitope binding on the envelope protein of flavivirus antigens captured with anti-flavivirus IgA in the presence of flavivirus positive sera. This test has sensitivity, specificity and speed comparable to the virus neutralization assay (VNT). C-ELISA is a flexible technology and can be applied in various ways. Minor modifications to this protocol result in C-ELISA-compliant secondary infection detection methods or C-ELISA-compliant methods that can be used for serotype-specific seroepidemiological studies and / or vaccine evaluation. Let's be done. Protocols developed for C-ELISA were presented using dengue lysate antigen and dengue specific monoclonal antibodies. The protocol can be used for other flaviviruses, and the Japanese encephalitis results show this.
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