abstract |
Antibody preparations with substantially homogeneous and non-sialylated glycoforms such as G0 and G2 are produced by enzymatic treatment, expression under certain conditions, use of specific host cells, and contact with serum . These antibody preparations include matrix metalloproteinases such as papain, ficin, bromelain, pepsin, MMP-7, neutrophil elastase (HNE), stromelysin (MMP-3) and macrophage elastase (MMP-12), and glycosyl It resists cleavage by proteases such as chelating modified enzymes. Antibody preparations with glycoforms that are substantially homogeneous and non-sialylated, and methods for testing glycosylation in antibodies are characterized by characterization of antibody properties and / or diseases or conditions characterized by increased protease activity It is useful in. |