| abstract |
Methods are provided for altering the N-glycosylation pattern of proteins in higher plants. The method includes introducing into the plant a recombinant construct that results in inhibition of expression of α1,3-fucosyltransferase (FucT) and β1,2-xylosyltransferase (XylT) in the plant. Using these constructs to inhibit or suppress the expression of both of these enzymes, and their isoforms, advantageously has a “humanized” N-glycosylation pattern without affecting plant growth and development. Endogenous heterologous proteins are produced. Provided are stably transformed higher plants having this protein N-glycosylation pattern. Also provided are glycoprotein compositions, including monoclonal antibody compositions, that have a substantially homogeneous glycosylation profile and are substantially homogeneous for the G0 glycoform. |