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publicationNumber JP-2009514535-A
titleOfInvention High-throughput screening assay for TRPM5 ion channel
abstract There is a need in the art for high-throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods have the disadvantages of lack of sensitivity, low throughput, and require a large labor force. The claimed method gives a quick result reading, has a high signal-to-noise background ratio, is easy to use, can be modified for automation and miniaturization, and the compound specifically modulates TRPM5 A fluorescence assay with optical readout is provided that provides a demonstration of modulation.
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