http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009511006-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2006-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2009511006-A |
| titleOfInvention | New selection system |
| abstract | The present invention relates to a method for producing a recombinant protein comprising the step of using a selection method other than antibiotics. Specifically, the present invention relates to a stable host / vector system based on the pyrC gene complementation designed to produce high levels of heterologous recombinant proteins in E. coli. The expression system of the present invention allows for the rapid selection of plasmid-containing cells during the cloning phase, resulting in a high degree of protein expression during the fermentation process. This system has a strong selection efficiency, especially during the induction time, thereby resulting in the selection of an almost homogeneous and stable plasmid-bearing cell population. Furthermore, the productivity of the culture is significantly better than that based on antibiotic resistance. This high vector stability, coupled with its high productivity, meets the requirements for heterologous protein production in E. coli to the industrial level. |
| priorityDate | 2005-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 506.