http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009504145-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B10-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1055 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2006-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2009504145-A |
| titleOfInvention | A method for evolution adapted to the continuous purpose of proteins in vitro |
| abstract | The present invention relates to a method for producing a mutant of a protein in the in vitro evolution method, which comprises the following steps: (A) a step of creating an in vitro expression system containing the following: (i) a protein Y to be mutated An encoding nucleic acid sequence S, (ii) a protein Y and / or a target molecule X 1 capable of binding to at least one variant Y, (iii) an RNA polymerase (Pol) capable of transcribing the nucleic acid sequence S (Iv) a reverse transcriptase (RT) capable of reverse transcribing the transcription product of the nucleic acid sequence S, wherein the target molecule X is coupled to Pol and the protein Y is coupled to RT or the target molecule X Is coupled to RT, and protein Y is coupled to Pol. (B) Translocation under the formation of mutant Y of protein Y and the nucleic acid sequence S ′ encoding it. Incubating the in vitro expression system from (A) under conditions that allow reverse transcription and translation and promote the formation of a variant Y with improved binding properties for the molecule of interest X, (C) in vitro comprising the steps of isolating variant Y with improved binding properties for binding to X and optionally characterizing step and / or isolating nucleic acid sequence variant S encoding Y The present invention relates to a method for producing a mutant of a protein in an evolution method. |
| priorityDate | 2005-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 147.