http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009284770-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bfdf49224a405be1f7b653c56ebad94 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
filingDate | 2008-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ec22adbe24edacb1f0f6ab0392b3b14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca9ac94897ad936cdc5d6dcda4132fbd |
publicationDate | 2009-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2009284770-A |
titleOfInvention | Method for detecting membrane protein of planktonic animal cell and screening method using the same |
abstract | Since the place where the introduced gene is incorporated cannot be specified in the floating animal cell, the expression level varies greatly depending on the place where the gene is incorporated. Therefore, in order to obtain a cell line highly expressed in a cell into which a membrane protein gene has been introduced, a detection system thereof is provided. Detection of membrane protein on the surface layer of suspension animal cells by immobilization of suspension animal cells, contact of a detection substance to the membrane protein, washing of the detection substance, and detection of the membrane protein by a predetermined anchor molecule Method. And a method for screening a floating animal cell expressing an introduced membrane protein gene by introducing a membrane protein gene into the floating animal cell, cell culture, detection of the cultured cell, and comparative selection of the expression level of the membrane protein in the cell. [Selection] Figure 4 |
priorityDate | 2008-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 146.