| abstract |
Disclosed is a composition that synergistically prevents proteolysis or modification of a sampled biological fluid. The composition uses a high concentration sulfonyl fluoride protease inhibitor in combination with at least one additional protease inhibitor of a different type, preferably a broad spectrum protease inhibitor, and a chelating agent. A preferred embodiment uses 10 mM AEBSF, 20 mM benzamidine, and EDTA as a chelator. The disclosed compositions may be used in combination with other protease inhibitors, for example, to further target its specificity, for example to additionally target acidic proteases. Additional protease inhibitors, reducing agents, stabilizers, and buffers are disclosed in devices for sampling or testing biological fluids for levels of peptides of interest, or methods therefor May be used in combination. [Selection] Figure 1 |