| abstract |
The present invention relates to a method for transforming mammalian cells, which can use a wide range of mammalian cells as host cells, has high gene introduction efficiency and high expression efficiency, and high selection efficiency of clones that highly express the target gene. I will provide a. The transformation method of the present invention comprises a first vector, which is an IR / MAR vector, and a second vector containing a promoter that functions in mammalian cells and a target gene encoding a secreted protein. Mammalian cells are co-transfected so that the molar ratio of the first vector to the vector is in the range of 0.3-5. [Selection figure] None |