abstract |
The present invention provides a method for isolating and identifying liver oval cells from the liver and a method for culturing liver oval cells in a single cell. A method for isolating and / or obtaining liver oval cells from a mammal, comprising the step of examining the expression of CD133, CD45 and Ter119, in particular three cell surface markers of CD133, CD45 and Ter119 are designated as CD133 + , CD45. Liver oval cells exhibiting a pattern of − and Ter119 − are isolated and / or obtained from the mammal. A method for culturing a liver oval cell, comprising culturing the obtained liver oval cell in the presence of a growth factor and an extracellular matrix. [Selection figure] None |