http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009142229-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1af8df51ce24ca931ae718fb747f6aa0 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2007-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b56f52a6fad70f6713a41a2ee0a69b8e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3c186c3556e7826f51c5a3adc3ba92b |
| publicationDate | 2009-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2009142229-A |
| titleOfInvention | Efficient hybridization method and kit |
| abstract | In hybridization with a probe nucleic acid immobilized on a solid phase, the efficiency of hybridization is reduced even if the target region of the probe nucleic acid is designed at a site other than the higher-order structure formation site of the nucleic acid sample. I got a good knowledge. This was found to be due to steric hindrance between adjacent nucleic acid samples or between the nucleic acid sample and the solid phase, which is caused by the nucleic acid sample forming a higher order structure at a position other than the hybridization site. This invention solves said subject and provides the method of improving the reaction efficiency of hybridization with the probe fix | immobilized on the solid-phase, and a nucleic acid sample. The present invention relates to a nucleic acid detection method and a nucleic acid in which a higher-order structure is destabilized using a nucleic acid sample containing a modified nucleoside, thereby mitigating these steric hindrances and increasing the efficiency of a hybridization reaction. A kit for detection is provided. [Selection] Figure 2 |
| priorityDate | 2007-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 108.