| abstract |
A method for producing hydroxyisoleucine or 2-amino-3-methyl-4-ketopentanoic acid is provided. Reduction of 2-amino-3-methyl-4-ketopentanoic acid or a salt thereof in the presence of a microorganism-derived enzyme that produces 4-hydroxyisoleucine from 2-amino-3-methyl-4-ketopentanoic acid A method for producing 4-hydroxyisoleucine or a salt thereof, comprising a step of producing 4-hydroxyisoleucine by reaction. A method for producing 2-amino-3-methyl-4-ketopentanoic acid or a salt thereof, comprising a step of reacting L-isoleucine in the presence of L-isoleucine dioxygenase and 4-hydroxy-L-isoleucine dehydrogenase. (2S, 3R, 4S) -4 from L-isoleucine in the presence of a bacterium having L-isoleucine dioxygenase activity that has been modified to reduce or eliminate the activity of 4-hydroxy-L-isoleucine dehydrogenase. -Method for producing hydroxy-L-isoleucine or a salt thereof. [Selection figure] None |