abstract |
A method for producing a male sterile or female sterile plant is provided. Also provided is a method for producing enantiomerically pure D-phosphinothricin (D-PPT). Transforming plant material with a polynucleotide encoding one or more enzymes that react with non-phytotoxic substances to produce phytotoxic substances, and thus regenerating the transformed material into plants, By applying the non-phytotoxic substance to the plant at the time of male and female gamete formation and / or maturity. This allows the non-phytotoxic substance to produce a phytotoxic substance that selectively prevents the gamete formation or renders the gamete selectively non-functional. Here, D-PPT is used as a non-phytotoxic substance, and D-amino acid oxidase is used as an enzyme. [Selection] Figure 1 |