| abstract |
To purify bacterial capsular polysaccharides. The precipitated bacterial capsular polysaccharide can be efficiently redissolved using alcohol as a solvent. The present invention provides a process for purifying bacterial capsular polysaccharide comprising the steps of (a) precipitating the polysaccharide and then (b) solubilizing the precipitated polysaccharide with ethanol. Include. CTAB can be used in step (a). Preferably, after hydrolysis and sizing, the resulting material can be conjugated to a carrier protein and formulated as a vaccine. Moreover, in the vaccine containing the saccharide | sugar derived from the cello group A and the cello group C, this invention provides the vaccine whose ratio (w / w) of MenA saccharide | sugar: MenC saccharide | sugar is> 1. [Selection] Figure 1 |