abstract |
A novel useful as a reagent in an immunoassay for detection and identification of antibodies against human immunodeficiency virus type 1 (HIV-1) M group, HIV-1 O group and human immunodeficiency virus type 2 (HIV-2) Provide antigen constructs and the like. An isolated HIV-1 group O env polypeptide obtained from HIV-1 isolate HAM112, and (a) each of HIV-1 group O env polypeptide and HIV-1 group M env polypeptide An antigen construct comprising one or more fusions, and (b) other antigen constructs containing additional group O sequences, particularly the gp41 IDR of isolate HAM112. Also, a polynucleotide sequence encoding the above, an expression vector containing the same, a host cell transformed thereby, and an immunoassay method and kit using the antigen construct of the present invention. [Selection figure] None |