http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009082017-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bd76b32e95e6adc5e05c442e43d496eb |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2007-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f712963f45798878648f6bad7ac11adf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cb8ab571f523e6e0f318863e3aede4a |
| publicationDate | 2009-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2009082017-A |
| titleOfInvention | Cell processing method for simultaneously detecting and evaluating localization and function of cell membrane proteins |
| abstract | It is possible to simultaneously detect and evaluate the location and function of a cell membrane protein that induces an increase in intracellular Ca 2+ concentration upon activation, and to screen for substances that promote or suppress the activity of the cell membrane protein. A cell processing method is provided. (1) Cell membrane protein A that induces an increase in intracellular Ca 2+ concentration fluorescently labeled with a fluorescent organic compound or fluorescent protein, or a cell membrane that induces an increase in intracellular Ca 2+ concentration fluorescently labeled with a fluorescent protein Gene A ′ encoding protein A is prepared. (2) A fluorescent probe protein B for detecting Ca 2+ that increases in concentration in the cell with the activation of the above-mentioned cell membrane protein A, or a gene B ′ encoding the same is prepared. (3) C is prepared by adding a nuclear translocation signal peptide to B, or C ′ is prepared by adding a gene encoding the nuclear translocation signal peptide to B ′. (4) A combination of either A + C or A ′ + C ′ is introduced or expressed in the same cell. [Selection] Figure 1 |
| priorityDate | 2007-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.