| abstract |
Disclosed is a sarcosine oxidase with reduced activity against proline obtained by modifying a protein having sarcosine oxidase activity by a protein engineering technique, a method for producing the same, and use of the sarcosine oxidase. In an amino acid sequence comprising a specific sequence, the 89th lysine is arginine, the 94th valine is glycine, the 204th methionine is alanine, the 213th serine is proline, and the 250th glutamic acid is glutamine. And a modified sarcosine oxidase substituted respectively. [Selection figure] None |