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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
filingDate 2007-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e11000071d7a56e44df7e570cb65350f
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publicationDate 2009-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2009019002-A
titleOfInvention Method for detecting On-chip phosphorylation by Michael reaction
abstract The present invention provides a method capable of comprehensively profiling the activity of various protein kinases, which is simple, excellent in specificity, and capable of high-throughput analysis. A method for detecting phosphorylation of a peptide or protein on a substrate, wherein the phosphorylated peptide or protein is treated with a base to remove a phosphate group by β and then modified with a thiol group. A method for detecting phosphorylation, which comprises performing a Michael addition reaction by reacting a rhodamine derivative compound. [Selection figure] None
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