http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008546405-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2006-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2008-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2008546405-A |
| titleOfInvention | Improved strategy for sequencing complex genomes using high-throughput sequencing techniques |
| abstract | Digesting the genome with at least one first restriction endonuclease; ligating at least one adapter to a first subset of restriction fragments; and an adapter ligation restriction fragment using a first primer combination. Selectively amplifying the first set of, wherein the first primer contains a first selection sequence (including 1-10 selective nucleotides) at the 3 ′ end of the primer sequence; Amplifying and repeating these steps using at least a second primer combination, wherein the primer contains a different second selection sequence, and repeating the amplified adapter ligation restriction fragment Fragmentation of each subset, whereby sequencing Generating a binding library, fragmenting, determining the nucleotide sequence of the fragments, aligning the sequences of the fragments in each of the libraries, thereby generating a contig, aligning; Repeating these steps for one second restriction endonuclease and / or further restriction endonuclease and aligning the contigs obtained for each of the second restriction endonuclease and / or further restriction endonuclease, Providing a sequence of the genome thereby aligning the genome sequence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013223502-A |
| priorityDate | 2005-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.