| abstract |
Genes encoding transcription factors (BMAL, Clock, NPAS, Per) that regulate core circadian oscillators and their regulatory targets (Rev-erbĪ±, Rev-erbĪ²) were found in adipose tissue. The circadian patterns of these genes were synchronized using restricted feeding. Circadian gene expression profiles were examined in mice after exposure to nuclear hormone receptor ligands (dexamethasone or thiazolidinedione) or 30% fetal calf serum, and human adipose stem cells differentiated in undifferentiated and adipocytes. . All three drugs induced a cyclic expression profile of representative circadian genes in human adipose stem cells. The circadian genes tested show a vibrational expression profile and are characterized by vertices and lowest points within the 24-28 hour phase. The circadian gene pattern was extended by using glycogen synthase kinase 3 beta inhibitor. Adjustments that extend and shorten circadian patterns can be used to affect weight gain or loss, respectively. |