http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008534016-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2006-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2008-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2008534016-A |
| titleOfInvention | Gene synthesis using pooled DNA |
| abstract | A method and system comprising a hierarchical partitioning step and a hierarchical assembly step for synthesizing one or more pieces of DNA having a desired sequence using pooled DNA. In the splitting step, the sequence of one or more DNA pieces having the desired nucleic acid sequence is recursively split into partially overlapping derived DNA pieces, and the derived DNA pieces are separated except after the final splitting step. Assign to multiple pools. At this time, overlapping overlapping derived DNA pieces are assigned to different pools. In the assembly stage, an oligonucleotide pool corresponding to the obtained DNA piece pool is obtained, and one or more DNA pieces having a desired sequence are assembled by overlap extension in the reverse order of the hierarchical division. This method embodiment combines the advantages of hierarchical assembly with the advantages of pooled oligonucleotides. [Selection figure] None |
| priorityDate | 2005-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 131.