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filingDate 2006-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2008-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2008529545-A
titleOfInvention Screening method for identifying protease-deficient mutants of microorganisms
abstract The present invention relates to a method for identifying a protease secretion deficient strain of a microorganism: Add a ciliate mutant to the gel; Incubating under conditions in which ciliate mutants secrete proteins; Separating the ciliate mutant from the gel; Where at least one substrate of at least one secreted protease of ciliates is contained in the gel and / or the gel itself is a substrate and / or the substrate is added later At least partly diffuses into the gel; The present invention also relates to a method for measuring protease activity acting on a substrate.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2012056817-A
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