| abstract |
Provided herein are methods and kits for making targeted therapeutics for treating a disease or condition. The therapeutic agent can target a patient specific disease marker. In one of these methods, the method includes obtaining a biological sample from a patient having a disease or condition or at risk of developing a disease or condition. In this particular method, the sample comprises a population of diseased cells and screens a library containing proteins linked to their cognate mRNAs to identify mRNA-protein pairs that bind to the diseased cells. One or more proteins are separated from the identified mRNA-protein pair, and the separated protein (s) are bound to a therapeutic agent. Part of the method further includes creating a library having proteins linked to its cognate mRNA. In some of these methods, generating the library comprises providing at least two candidate mRNA molecules, each mRNA molecule comprising a cross-linking agent, to generate at least one translated protein. Translating at least two of the candidate mRNA molecules and linking at least one of said candidate mRNA molecules to its corresponding translated protein via said cross-linking agent forming at least one cognate pair Including that. |