| abstract |
The present invention uses vectors containing expression cassettes encoding one or more protein / peptide antigens and / or adjuvants, as well as immunization / vaccination, or animal immune systems, including humans, using recombinase-mediated integration. It relates to an improved method for stimulating. The adjuvant is in particular a cytokine such as GMCSF, Flt3L, interleukin, and the adjuvant can also be encoded by DNA. Adjuvants known to boost immune responses according to DNA vaccination. Furthermore, the vector contains one or more sites recognized by recombinase / transposase. The recombinase / transposase catalyzes the integration of the vector into the genome of the transfected cell. |