http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008517602-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2005-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2008-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2008517602-A |
| titleOfInvention | Repair of nucleic acids to improve amplification |
| abstract | Methods and compositions for repairing polynucleotides are provided so that they can be efficiently synthesized with improved fidelity and yield, for example, in amplification reactions. This involves the use of a reaction mixture comprising a ligase and a cofactor selected from NAD + or ATP and incubating the polynucleotide with the reaction mixture in the absence of endonuclease VI. The reaction mixture can further comprise an AP endonuclease and a polymerase. These enzymes may be selected according to their ability to withstand high temperatures so that they can be included in the amplification mixture. If an enzyme that is not thermophilic can be used, the reaction mixture can be used prior to the polynucleotide synthesis reaction. The repair reaction is unaffected by time in terms of seconds, minutes or hours of incubation in the enzyme mixture. |
| priorityDate | 2004-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 396.