http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008507965-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2005-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2008-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2008507965-A |
| titleOfInvention | Methods for producing highly sensitive endonucleases, novel endonuclease preparations and uses thereof |
| abstract | The present invention relates to methods for producing recombinant endonucleases with high sensitivity, endonuclease preparations obtained by such methods, and their use for the detection of mismatches in particular. [Sequence Listing] SEQUENCE LISTING <110> GENOPLANTE-VALOR INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE BENDAHMANE, Abdelhafid STURBOIS, Benedicte TRIQUES, Karine CABOCHE, Michel <120> METHOD FOR PRODUCING HIGHLY SENSITIVE ENDONUCLEASES, NOVEL PREPARATIONS OF ENDONUCLEASES AND USES THEREOF. <130> MJP / bv1516-19 <150> PCT / EP2004 / 009159 <151> 2004-07-30 <150> PCT / EP2004 / 009166 <151> 2004-07-30 <160> 25 <170> PatentIn version 3.3 <210> 1 <211> 2640 <212> DNA <213> Arabidopsis thaliana <220> <221> misc # feature <223> ENDO 1 Gene At1g11190 <400> 1 aaattcgatg aggttgttat agacaagaga agacattttt atacaaaaga gtttatcatt 60 atataagttt caaactttga agatatggca tcggctttta gatcatccac gaggttgatt 120 cttgtattag gtatactgat tttgtgttcg gtttcttctg tccgaagctg gagcaaagaa 180 ggtcatattc ttacttgtag aattgctcag gtaattaagt taatgatcta ttgtttgaag 240 caactatttt ggttattctt gtcttatata tgtattagtg agatatacct acaaattttt 300 aattaggatt gacttttaaa ttgctatacg ttaccatgcc taacatctca tgtagatgat 360 catgaataca aacatgtcta atggcatatc aaattccaag tttttttggt agagatctga 420 gtcatttgac cgttataaga ttcataacaa aagttcgtat gtgtgtgttt ttgtggtgtg 480 accagaatct tttagaagcc ggaccagcac atgtagtaga gaatctgtta ccggattacg 540 tgaaaggaga tttatcagca ttgtgtgtgt ggcctgacca gatccgacat tggtacaagt 600 atcgttggac cagccatctc cattacatcg acactcccga ccaagcctgc tcttacgaat 660 actctagtaa gtcacaaccg agacattttc agataacctt aatccgtttt ctaattatct 720 tgaaccggag ttaaccaaaa aatcaattac aaataccaaa ccggattaaa aacaggggat 780 tgtcatgatc aacatggatt gaaggatatg tgtgtggatg gagcaatcca gaatttcacg 840 tctcagcttc agcattacgg tgaaggaaca tctgatcgta gatgtatgtc atcattttca 900 tttatttcat ataatgatga tatccaaagt gtaactgcgt attttgtatt ttgatgcata 960 acttaagttt ttaaaattat aatatatcct tgttcaatca catagataac atgaccgaag 1020 cccttttgtt cttgtctcat ttcatgggag atattcatca ggtttattac tcatcatcga 1080 ttcatttcac acctccacac atatagctct atttccatgt taaatattta attaacatgg 1140 tttttttttt tttccttaaa aagccgatgc atgtgggatt cacaagtgat gaaggaggaa 1200 acacgataga tttacgttgg tacaaacaca aatccaatct acatcatgta agcttcttct 1260 tttgtctctt tcaactttaa atttcatcat gaaaacaaaa aaaaaattaa cgaaggaaac 1320 aaaatatgta ggtatgggat agagagatca ttctcacggc tctaaaagaa aactacgaca 1380 agaacttgga tcttctccaa gaggatcttg agaagaacat caccaatgta atagacacta 1440 atttattcat attttactat aattttaaga atctttataa tggttatcat atattaggga 1500 ttatggcacg acgatctatc ttcgtggaca gaatgcaacg atcttatcgc ttgtccacac 1560 aagtaagttt taaattactt ggtttaagat tggcttgacg ctcgtttgaa gctagctaca 1620 aattttgata ctttttctgg tccaaaaatc ttacaaagat actgaaaata aaataatagg 1680 ttttaaactt ttaatttatt tggagttgga taggattaag tttcactaac ttccaattca 1740 aagtcaatta atagtagttt accatgatta gtgggttgac taatgtacca tatatattac 1800 cttatatcac atcttatttc cgatgtgaga tttcttatga aacataatta gactcgaacc 1860 ttttgtgttt cgatatatgt agtgtattca tgatcagaat cttattaagt ttacaactga 1920 aaactaaaat attaacatca taattataga ttcttaagta ggttttgttt gggtggagaa 1980 aatatccaat ttcgaataac attatataaa atattgaact aattttaatt gtatacgcag 2040 gtatgcttca gagagtataa agttagcttg taaatgggga tacaaaggcg tcaagtctgg 2100 tgaaacgtta tcaggtacgt tgtttgcttc ttctttttct cgtacgctaa caaaaatatt 2160 taaaaataaa cccgaccaaa tgaagtttaa ttaatcggat taatgatttt taatagtcac 2220 tacttttttt gtgtgggata tatgactgtc taatatataa ttttataaga aagctaaagg 2280 atttgtttaa taatttccga taaataattt tgcagaagaa tatttcaata caaggttgcc 2340 aatagtgatg aagagaatag ttcagggagg agttagacta gccatgatac taaaccgggt 2400 ttttagtgac gatcatgcta ttgctggtgt tgctgccact tgaaccaaac ccgacatacc 2460 ggggcatcaa agcatttgat taagagatta tttgatacat tcacaaaatt aattaaggct 2520 gatcagacat tcttttcttt tagtagcttt atctatgtga cagctaatgc ctgtggactg 2580 ctttgtttag aagtgtttag cattagatca tatgctaatt caatgttatt aattcatcgt 2640 <210> 2 <211> 305 <212> PRT <213> Arabidopsis thaliana <220> <221> misc # feature <223> Protein ENDO 1 At1g11190 <400> 2 Met Ala Ser Ala Phe Arg Ser Ser Thr Arg Leu Ile Leu Val Leu Gly 1 5 10 15 Ile Leu Ile Leu Cys Ser Val Ser Ser Val Arg Ser Trp Ser Lys Glu 20 25 30 Gly His Ile Leu Thr Cys Arg Ile Ala Gln Asn Leu Leu Glu Ala Gly 35 40 45 Pro Ala His Val Val Glu Asn Leu Leu Pro Asp Tyr Val Lys Gly Asp 50 55 60 Leu Ser Ala Leu Cys Val Trp Pro Asp Gln Ile Arg His Trp Tyr Lys 65 70 75 80 Tyr Arg Trp Thr Ser His Leu His Tyr Ile Asp Thr Pro Asp Gln Ala 85 90 95 Cys Ser Tyr Glu Tyr Ser Arg Asp Cys His Asp Gln His Gly Leu Lys 100 105 110 Asp Met Cys Val Asp Gly Ala Ile Gln Asn Phe Thr Ser Gln Leu Gln 115 120 125 His Tyr Gly Glu Gly Thr Ser Asp Arg Arg Tyr Asn Met Thr Glu Ala 130 135 140 Leu Leu Phe Leu Ser His Phe Met Gly Asp Ile His Gln Pro Met His 145 150 155 160 Val Gly Phe Thr Ser Asp Glu Gly Gly Asn Thr Ile Asp Leu Arg Trp 165 170 175 Tyr Lys His Lys Ser Asn Leu His His Val Trp Asp Arg Glu Ile Ile 180 185 190 Leu Thr Ala Leu Lys Glu Asn Tyr Asp Lys Asn Leu Asp Leu Leu Gln 195 200 205 Glu Asp Leu Glu Lys Asn Ile Thr Asn Gly Leu Trp His Asp Asp Leu 210 215 220 Ser Ser Trp Thr Glu Cys Asn Asp Leu Ile Ala Cys Pro His Lys Tyr 225 230 235 240 Ala Ser Glu Ser Ile Lys Leu Ala Cys Lys Trp Gly Tyr Lys Gly Val 245 250 255 Lys Ser Gly Glu Thr Leu Ser Glu Glu Tyr Phe Asn Thr Arg Leu Pro 260 265 270 Ile Val Met Lys Arg Ile Val Gln Gly Gly Val Arg Leu Ala Met Ile 275 280 285 Leu Asn Arg Val Phe Ser Asp Asp His Ala Ile Ala Gly Val Ala Ala 290 295 300 Thr 305 <210> 3 <211> 28 <212> DNA <213> Artificial sequence <220> <223> Primer 4-960 <400> 3 gtgtttgtcc agtaatagtg tcagcata 28 <210> 4 <211> 26 <212> DNA <213> Artificial sequence <220> <223> Primer 4-721 <400> 4 aggaacctga gaaaagactc gccagc 26 <210> 5 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> CEL N Terminal <400> 5 tatcgttcta gagggaatga cgcgattata ttctgtgttc 40 <210> 6 <211> 27 <212> DNA <213> Artificial sequence <220> <223> CEL C Terminal <400> 6 tatctgaatt catgccaaag aatgatc 27 <210> 7 <211> 50 <212> DNA <213> Artificial sequence <220> <223> CEL C terminal 8 His <400> 7 aattcaatgg tgatggtggt gatggtgatg tgccaaagaa tgatctgcgg 50 <210> 8 <211> 25 <212> DNA <213> Artificial sequence <220> <223> Primer (R21) <400> 8 gacatatgga ctacagaagc ttggg 25 <210> 9 <211> 25 <212> DNA <213> Artificial sequence <220> <223> Primer (R22) <400> 9 gttcacgggt cacatcatgc attcc 25 <210> 10 <211> 22 <212> DNA <213> Artificial sequence <220> <223> Primer 4m118 <400> 10 ttggttggac ttcactttga gc 22 <210> 11 <211> 22 <212> DNA <213> Artificial sequence <220> <223> Primer 4m984 <400> 11 cacaacaatc agcaatgaca gc 22 <210> 12 <211> 23 <212> DNA <213> Artificial sequence <220> <223> Primer 4-347 <400> 12 gtgattgctc cacctccgcc acc 23 <210> 13 <211> 30 <212> DNA <213> Artificial sequence <220> <223> Primer 4-134 <400> 13 tacagcgatt gatataatat aaaattatcc 30 <210> 14 <211> 27 <212> DNA <213> Artificial sequence <220> <223> Primer le 2462 <400> 14 tgatattgtc gtgcaatatg atgaaac 27 <210> 15 <211> 25 <212> DNA <213> Artificial sequence <220> <223> Primer le 3082 <400> 15 atacctattt agcccacttg gacac 25 <210> 16 <211> 27 <212> DNA <213> Artificial sequence <220> <223> Forward Primer for ENDO5 <400> 16 aaggatccga aagctctgtg tttcaga 27 <210> 17 <211> 28 <212> DNA <213> Artificial sequence <220> <223> Reverse Primer for ENDO5 <400> 17 ggagttgtta cgtgggttct caaggatc 28 <210> 18 <211> 28 <212> DNA <213> Artificial sequence <220> <223> Forward Primer for ENDO4 <400> 18 ctggatccct gtttttaact ttggaaag 28 <210> 19 <211> 26 <212> DNA <213> Artificial sequence <220> <223> Reverse Primer for ENDO4 <400> 19 ggatgttcaa gtgattctcc tggatc 26 <210> 20 <211> 27 <212> DNA <213> Artificial sequence <220> <223> Forward Primer for ENDO3 <400> 20 aaggatccat tcgacaaact ttgtaac 27 <210> 21 <211> 28 <212> DNA <213> Artificial sequence <220> <223> Reverse Primer for ENDO3 <400> 21 agagtggtct tgggaatatt tatctcag 28 <210> 22 <211> 26 <212> DNA <213> Artificial sequence <220> <223> Forward Primer for ENDO2 <400> 22 acggatccca tttcaaagaa ctctga 26 <210> 23 <211> 26 <212> DNA <213> Artificial sequence <220> <223> Reverse Primer for ENDO2 <400> 23 gaccaatcat tatgctgtaa cttcag 26 <210> 24 <211> 25 <212> DNA <213> Artificial sequence <220> <223> Forward Primer for ENDO1 <400> 24 caggatccaa gtttcaaact tgaag 25 <210> 25 <211> 26 <212> DNA <213> Artificial sequence <220> <223> Reverse Primer for ENDO1 <400> 25 cggtatgtcg ggtttggttc aagtgg 26 |
| priorityDate | 2004-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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Total number of triples: 313.