| abstract |
The present invention is directed to lipid-based formulations for delivering, eg, introducing nucleic acid lipid particles into cells, and assays to optimize the delivery efficiency of such lipid-based formulations. Nucleic acid lipid particles are interfering RNA molecules, cationic lipids having an alkyl side chain of about 10 to about 20 carbon atoms with one or more sites of unsaturation, non-cationic lipids, and polyethylene glycol (PEG) lipids Include complex lipids that prevent particle aggregation, such as complexes or polyamide (ATTA) complexes. |