| abstract |
PROBLEM TO BE SOLVED: To observe a change in the speed of movement of a biomolecule or the like by a single molecule fluorescence analysis technique such as fluorescence correlation spectroscopy (FCS), and to investigate the interaction of the biomolecule etc. To detect well or with high sensitivity while reducing costs. The method of the present invention comprises a fluorescently labeled first molecule, a second molecule to which a predetermined modifying group is attached, and a third molecule that specifically binds to the predetermined modifying group. Based on the fluorescence intensity of the reaction mixture solution, the index value of the Brownian motion speed of the first molecule is calculated, compared to when the Brownian motion speed of the first molecule is liberated alone. When it is determined that the second molecule is late, it is determined that the second molecule is bound to the first molecule. [Selection] Figure 2 |