| abstract |
An object of the present invention is to fermentatively produce 3-carboxy-cis, cis-muconic acid from terephthalic acid on an industrial scale. [MEANS FOR SOLVING PROBLEMS] On a chromosomal DNA of a microbial cell, (a) a specific amino acid sequence; or (b) an amino acid in which one or more amino acids are deleted, substituted, added and / or inserted in the specific amino acid sequence A gene-disrupted strain of a protocatechuic acid 4,5-ring-cleaving enzyme, wherein the gene encoding the amino acid sequence having protocatechuic acid 4,5-ring-cleaving activity is disrupted; the Tph gene and protocatechuic acid 3,4- A recombinant plasmid containing a dioxygenase gene; a transformant obtained by introducing the recombinant plasmid into the disrupted strain; and culturing the transformant in the presence of terephthalic acid, 3 -Method for producing carboxy-cis, cis-muconic acid and / or 3-carboxymuconolactone. [Selection figure] None |