| abstract |
An object of the present invention is to provide an immobilized protein in which orientation control is immobilized at one amino terminal. [MEANS FOR SOLVING PROBLEMS] An amino acid sequence having no lysine and cysteine residues represented by the general formula S1-R1-R2 [wherein the sequence represents a sequence from the amino terminal side to the carboxy terminal side, The sequence of the S1 portion may not be present, and if present is a spacer sequence composed of amino acid residues other than lysine and cysteine residues; The sequence of the R1 portion is the sequence of the protein to be immobilized, and is a sequence characterized by not containing lysine residues and cysteine residues; The sequence of R2 portion may not be present, and if present, is a spacer sequence composed of amino acid residues other than lysine and cysteine residues] An immobilized protein, wherein one amino terminal end of the protein is bound to an immobilization carrier through a group. [Selection figure] None |