http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008259510-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0950e9df7f0e1b73efee1bda859951ad |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-416 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2008-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8911bb36f00e91bbc99cd1db5e84822 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab569d507bac06495aa1e47bf7f6718c |
| publicationDate | 2008-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2008259510-A |
| titleOfInvention | Method for detecting target nucleic acid sequence |
| abstract | The present invention provides a LAMP amplification product that enables simple and short-time nucleic acid detection, and a method for detecting a target nucleic acid sequence present in the LAMP amplification product. A stem comprising a complementary sequence portion at both ends and a target sequence portion existing between the complementary sequence portions, and a double-stranded portion and another loop-like single-stranded portion by hybridization. A step of preparing a measurement nucleic acid having an AND loop structure, a step of preparing a probe nucleic acid having one end bonded to the surface of a solid phase substrate, and a step of hybridizing a target sequence portion of the measurement nucleic acid to the probe nucleic acid And detecting the presence or absence of the measurement nucleic acid hybridized to the probe nucleic acid, and when hybridized, the double-stranded part of the measurement nucleic acid extends in a direction away from the solid surface. Thus, the target nucleic acid sequence that sets the orientation of the 5 ′ → 3 ′ sequence in both the probe nucleic acid and the target sequence portion The method of detection. [Selection] Figure 3 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020065488-A |
| priorityDate | 2003-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.