| abstract |
[PROBLEMS] To achieve a column operation for a packed bed at a high flow rate that achieves both high adsorption capacity and high flow rate processability, and because of the high adsorption capacity, the amount of protein that can be purified at one time is large, and the purification rate is remarkably improved. A novel filler for a packed bed that can be provided. SOLUTION: Porous particles obtained by polymerizing an unsaturated synthetic monomer and having a pore volume of 50% by volume or more, and the inside of the pore is shared by a water-soluble polymer having no crosslinked structure. The high adsorption capacity and the improvement of the purification rate are achieved by a novel filler characterized in that it is immobilized and filled by a bond and has an affinity functional group covalently bonded to the water-soluble polymer site. [Selection figure] None |