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filingDate 2007-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2008-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2008220249-A
titleOfInvention Method for purification of earthworm-derived serine protease
abstract Provided is a method capable of easily, quickly and highly purifying earthworm-derived serine protease with high specific activity. As a new use of earthworm-derived serine protease, a detergent containing earthworm-derived serine protease is provided. To do. A method for purifying earthworm-derived serine protease, comprising dipping earthworms in an aqueous solution containing ethanol, 2-propanol or acetone or an organic solvent thereof at 20% or more for at least one day, and buffering the earthworms There is provided a purification method comprising a crushing step of homogenizing in a liquid to prepare a homogenate, and a recovery step of centrifuging the homogenate to remove precipitates and recovering earthworm-derived serine protease as a supernatant. [Selection figure] None
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