| abstract |
Based on the knowledge obtained from the analysis results of GAK and PP2A B′α, a means for elucidating a signal transduction mechanism related to these, and a medicine having a new mechanism of action based on an action function revealed by the means And the development method. PP2A B′α variant in which threonine phosphorylated by GAK is substituted or deleted by another amino acid, or a fragment thereof containing the substituted or deleted portion; PP2A and phosphorylation In a phosphatase reaction system containing a substance, the substance having reduced degree of dephosphorylation by coexistence with the PP2A B′α mutant or a fragment thereof is identified as a dephosphorylation target of PP2A. A method for screening an oxidation target; a method for screening a substance capable of regulating the transcriptional activity of p53, including evaluating whether a test substance can regulate the expression or activity of a constituent factor of PP2A. [Selection figure] None |